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iscript gdna clear cdna synthesis kit  (Bio-Rad)


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    Bio-Rad iscript gdna clear cdna synthesis kit
    Iscript Gdna Clear Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 49689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+gdna+clear+cdna+synthesis+kit/iScript+cDNA+Synthesis+Kit/pmc13084646-79-1-7
    Average 99 stars, based on 49689 article reviews
    iscript gdna clear cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Systemic delivery of anti-sense oligonucleotide targeting α-synuclein for treatment in a mouse model of multiple system atrophy
    Article Snippet: RNA was extracted from the remaining homogenized sample ( N = 4 for each group of animals) using RNAeasy kit (Qiagen) and quantified by spectrophotometry readings. .. For cDNA synthesis, 500 ng of total RNA was reverse transcribed using iScript gDNA Clear cDNA Synthesis kit (BioRad). .. Real time-PCR (RT-PCR) was performed using IDT primetime gene expression master mix (IDT) with primer/probe designed for mouse myelin basic protein (IDT, Mm.PT.58.28532164), and human αSyn (IDT, Hs.PT.58.912923).

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1
    Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An iScript gDNA Clear cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to convert RNA to cDNA, which was amplified using PowerTrack SYBR Green MasterMix (Applied Biosystems, Waltham, MA). qPCR was performed in QuantStudio 3 thermal cycler (Applied Biosystems). ..

    Article Title: Enforced ZFP281 expression delays breast cancer initiation and can provide lifelong protection against breast cancer metastasis
    Article Snippet: Total RNA was extracted using the RNeasy Plus Micro kit (Qiagen, 74034). .. Reverse transcription was performed using the iScript gDNA clear cDNA synthesis kit (BioRad, 1725035). .. Relative expression levels of RNA were determined using PowerUP SYBR TM Green master Mix (Applied Biosystems, A25776) by a QuantStudio 3 Real-Time PCR System (Applied Biosystems, A28567).

    Article Title: Optimized TadA-derived base editors efficiently manipulate mRNA splicing by A-to-G and C-to-K editing in potato.
    Article Snippet: Total RNA was extracted from young potato leaves using an RNAprep Pure Plant Kit (TIANGEN, Beijing, China) following the manufacturer's instructions. .. First‐strand cDNA was synthesized from 1 μg of total RNA using an iScript gDNA Clear cDNA Synthesis Kit (Bio‐Rad, California, USA). .. PCR was performed using Phanta Max Super‐Fidelity DNA Polymerase (Vazyme, #P505, Nanjing, China) with two pairs of primers, DL1‐F/R and PDS‐intronF1/R1 to detect StDL1 and StPDS, respectively (Table S13).

    Article Title: Peptide-Mediated Delivery of α-Synuclein Targeting ASO: Pharmacokinetics, Safety, and CNS Efficacy in a Synucleinopathy Model
    Article Snippet: The purity and concentration of RNA were assessed 304 using the Invitrogen Nanodrop One Spectrophotometer. .. For cDNA synthesis, 500 ng of total 305 RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (BioRad). .. 306 Jo urn al Pr e-p roo f 15 Real-time PCR (RT-PCR) was performed using the IDT PrimeTime Gene Expression Master 307 Mix (IDT) with primer/probe sets specific for human α-synuclein (IDT, Hs.PT.58.912923).

    Article Title: TWEAK-induced alternative NF-κB signaling regulates Cxcl10 and Mymx to promote myoblast-to-myotube fusion and muscle regeneration.
    Article Snippet: Total RNA was isolated from primary myotubes and C2C12 myotubes using RNAzol RT (R4533, Millipore Sigma) according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was generated using iScript gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad Laboratories) following manufacturer’s protocol. .. RT-qPCRs were performed using SsoAdvanced Universal SYBR Green Supermix (1725271, Bio-Rad Laboratories) on a QuantStudio 6 Pro Real-Time PCR System (ThermoFisher Scientific).

    Reverse Transcription:

    Article Title: Systemic delivery of anti-sense oligonucleotide targeting α-synuclein for treatment in a mouse model of multiple system atrophy
    Article Snippet: RNA was extracted from the remaining homogenized sample ( N = 4 for each group of animals) using RNAeasy kit (Qiagen) and quantified by spectrophotometry readings. .. For cDNA synthesis, 500 ng of total RNA was reverse transcribed using iScript gDNA Clear cDNA Synthesis kit (BioRad). .. Real time-PCR (RT-PCR) was performed using IDT primetime gene expression master mix (IDT) with primer/probe designed for mouse myelin basic protein (IDT, Mm.PT.58.28532164), and human αSyn (IDT, Hs.PT.58.912923).

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Article Title: Enforced ZFP281 expression delays breast cancer initiation and can provide lifelong protection against breast cancer metastasis
    Article Snippet: Total RNA was extracted using the RNeasy Plus Micro kit (Qiagen, 74034). .. Reverse transcription was performed using the iScript gDNA clear cDNA synthesis kit (BioRad, 1725035). .. Relative expression levels of RNA were determined using PowerUP SYBR TM Green master Mix (Applied Biosystems, A25776) by a QuantStudio 3 Real-Time PCR System (Applied Biosystems, A28567).

    Article Title: Peptide-Mediated Delivery of α-Synuclein Targeting ASO: Pharmacokinetics, Safety, and CNS Efficacy in a Synucleinopathy Model
    Article Snippet: The purity and concentration of RNA were assessed 304 using the Invitrogen Nanodrop One Spectrophotometer. .. For cDNA synthesis, 500 ng of total 305 RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (BioRad). .. 306 Jo urn al Pr e-p roo f 15 Real-time PCR (RT-PCR) was performed using the IDT PrimeTime Gene Expression Master 307 Mix (IDT) with primer/probe sets specific for human α-synuclein (IDT, Hs.PT.58.912923).

    Quantitative RT-PCR:

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1
    Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An iScript gDNA Clear cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to convert RNA to cDNA, which was amplified using PowerTrack SYBR Green MasterMix (Applied Biosystems, Waltham, MA). qPCR was performed in QuantStudio 3 thermal cycler (Applied Biosystems). ..

    SYBR Green Assay:

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1
    Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An iScript gDNA Clear cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to convert RNA to cDNA, which was amplified using PowerTrack SYBR Green MasterMix (Applied Biosystems, Waltham, MA). qPCR was performed in QuantStudio 3 thermal cycler (Applied Biosystems). ..

    Amplification:

    Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1
    Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An iScript gDNA Clear cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to convert RNA to cDNA, which was amplified using PowerTrack SYBR Green MasterMix (Applied Biosystems, Waltham, MA). qPCR was performed in QuantStudio 3 thermal cycler (Applied Biosystems). ..

    Synthesized:

    Article Title: Optimized TadA-derived base editors efficiently manipulate mRNA splicing by A-to-G and C-to-K editing in potato.
    Article Snippet: Total RNA was extracted from young potato leaves using an RNAprep Pure Plant Kit (TIANGEN, Beijing, China) following the manufacturer's instructions. .. First‐strand cDNA was synthesized from 1 μg of total RNA using an iScript gDNA Clear cDNA Synthesis Kit (Bio‐Rad, California, USA). .. PCR was performed using Phanta Max Super‐Fidelity DNA Polymerase (Vazyme, #P505, Nanjing, China) with two pairs of primers, DL1‐F/R and PDS‐intronF1/R1 to detect StDL1 and StPDS, respectively (Table S13).

    Generated:

    Article Title: TWEAK-induced alternative NF-κB signaling regulates Cxcl10 and Mymx to promote myoblast-to-myotube fusion and muscle regeneration.
    Article Snippet: Total RNA was isolated from primary myotubes and C2C12 myotubes using RNAzol RT (R4533, Millipore Sigma) according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was generated using iScript gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad Laboratories) following manufacturer’s protocol. .. RT-qPCRs were performed using SsoAdvanced Universal SYBR Green Supermix (1725271, Bio-Rad Laboratories) on a QuantStudio 6 Pro Real-Time PCR System (ThermoFisher Scientific).



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